POSEIDON PROJECT

The POSEIDON project is an excellent example of a strategic application of ARC technology whose performances open new road for the development of innovative and smart products.

Project reference: 644669 Start/End: Gen 2015-Dic 2017 Total cost: EUR 4.068.781 EU contribution: EUR 4.068.781 Programmeacronym: H2020-ICT-2014-1 Subprogramme area: ICT-26-2014 Photonics KET Contract type: Research and Innovation Actions

The objective of the POSEIDON project is to develop a SPR-based biosensing platform for the detection of L. pneumophila bacteria, with high sensitivity and high specificity, translating the results obtained as experimental proof of concept into an operating automated prototype usable in industrially relevant settings and by untrained personnel.

The following challenges will be pursued throughout the development of the project.

  • High sensitivity and low detection limit
  • Selectivity towards target pathogen detection in order to avoid both false-positive and false-negative results
  • Short analysis times
  • Ease of use, possibility of on-site monitoring and automation of the sample manipulation and detection procedure.
  • Efficient delivery of the bacteria: cells should remain intact throughout the whole fluid transportation system in the device, and should not adhere to the fluidic piping and microfluidic channels, so that virtually all of the bacteria cells in the sample are delivered to the sensing unit.
  • The size of the device should allow samples to be analyzed at the point of need rather than in a separate laboratory, allowing reduction of cost per single measurement and increase in throughput.

 

 

 

The POSEIDON project adopts a multidisciplinary approach involving key enabling technologies (KET) in photonics, aiming at the exploitation of the Surface Plasmon Resonance (SPR) phenomenon to develop a fully automated platform for fast optical detection of L. pneumophila pathogens.

SPR sensors provide an extremely sensitive and versatile tool for miniaturized label-free sensing platforms integrated into lab-on-chip systems for potential applications in environmental monitoring, biotechnology, medical diagnostics, drug screening, food safety and security.

Detection and investigation of viruses, bacteria and eukaryotic cells is nowadays becoming a rapidly growing field in SPR biosensing, but the detection was only achieved in laboratory settings. In this project an innovative sensing device architecture will be used to create a platform to yield reliable measurement readouts of legionella bacterial cells that would be driven and entrapped on a custom sensing surface specifically designed with opportune positive and negative controls.

The detection platform will be implemented as a prototype in which water and air samples are sequentially concentrated, injected into a microfluidic system, and delivered to the SPR sensor for analysis. The system will be designed to allow for its future integration in water distribution and HVAC (heating, ventilation and air conditioning) for prevention of L. pneumophila outbreaks.

The POSEIDON project aims to address new solutions through several activities ranging from research to piloting. The following schematic overview of POSEIDON project, was depicted visualizing the different system components and the partners’ involvement in their development (see legend) and the connection in the workflow.

for more information about the project or possible new collaborations please contact us at: poseidonprojects@arc-projects.it and visit the project web page: www.poseidonproject.eu

PROJECT DETAILS

Project partners:

Uppsala University (Sweden)

Uppsala University (Sweden)

Uppsala University, founded in 1477, is a comprehensive university with nine faculties, about 40,000 students, and c. 6,000 employees. The university has c. 600 full professors, 22% of which are women…
CatLab (Spain)

CatLab (Spain)

Catlab is a Clinical Analysis Laboratory that was born from the merger of the laboratories of the Hospital UniversitariMútua de Terrassa (HUMT) and the ConsorciSanitari de Terrassa (CST). In June 2012 the laboratory of …
ProtoLab s.r.l. (Italy)

ProtoLab s.r.l. (Italy)

Protolab provides its customers with a decade of expertise in the system integration, starting from the design of both Hardware and Software, to the realization of complete solutions tailored to customer needs.
Metrohm Applikon (Netherlands)

Metrohm Applikon (Netherlands)

Metrohm Applikon is a member of the Metrohm A.G. group and is a company that specializes in on-line process analysis. The company was founded in 1984 and has since then focussed …
Clivet s.r.l. (Italy)

Clivet s.r.l. (Italy)

Clivet is a European leading company that designs, produces and distributes systems for cooling, heating, air ventilation & purification with a wide range of solutions for residential, commercial and industrial…

 

Project reference: 644669

Start/End: Gen 2015-Dic 2017

Total cost: EUR 4.068.781

EU contribution: EUR 4.068.781

Programme acronym: H2020-ICT-2014-1

Subprogramme area: ICT-26-2014 Photonics KET

Contract type: Research and Innovation Actions